A ratiometric fluorogenic nanoprobe for real-time quantitative monitoring of lysosomal pH

Lysosomes are known as key players in cellular signalling and act terminal degradation stations involved a multitude of processes. Being highly influential physiological factor, pH is essential the regulation lysosome-mediated pathological Aberrant fluctuations related to lysosomal dysfunction that correlates storage diseases neurodegenerative disorders. As such, real-time quantitative monitoring (pHL) crucial for gaining insight into but challenging by lack effective lysosome-specific probes with high signal fidelity. Toward this end, we have proposed fluorogenic nanoprobe (TR-MP) reliable ratiometric measuring pHL. It fabricated rational manipulation fluorescence resonance energy transfer (FRET) tailorable nanoplatform. The consists biocompatible silica nanoparticles assembled pH-sensitive rhodamine derivative (RDM-TEOS) an acceptor aggregation-induced emission (AIE) fluorophore (TPE-OMe) donor ensure efficiency. Further equipped cell-penetrating facilitator morpholine enable cell-internalization lysosome affinity TR-MP. Results show TR-MP can quantitatively measure range 3.0 – 7.0 detect pHL live cells under various stimuli, well monitor during apoptosis.